Cover and Seal
Instruction and Parameters
{
"op": "seal",
"object": container
}
{
"op": "unseal",
"object": container
}
{
"op": "cover",
"object": container,
"lid": "standard" | "universal" |
"low_evaporation"
}
{
"op": "uncover",
"object": container
}
Some instructions, such as the thermocycle
or incubate
instruction must be preceded by a seal
or cover
instruction.
On the other hand, if a container has a seal or a cover, it must be unseal
ed or uncover
ed before performing any action on the aliquots within. Autoprotocol-python makes this easy by automatically checking the cover status of a container before certain instructions and inserting a cover/seal
(uncover/unseal
) instruction where required.
Additionally, if executing a run as a package , a seal_on_store
function is called at the end of the run which will apply a cover/seal
instruction to all containers destined for storage, which are not already covered or sealed.
Some instructions can be run on covered or sealed containers, which are listed below:
Instruction | No cover? | Covered? | Sealed? |
---|---|---|---|
pipette | yes | ||
thermocycle | yes | ||
absorbance | yes | yes | |
fluorescence | yes | yes | |
luminescence | yes | yes | |
incubate | yes | yes | |
gel_separate | yes | ||
centrifugation | yes | yes | yes |
For more information on covers and seals, see the Containers section.
Device
In the case of PCR plates, the seal will be applied by an ALPS-3000 automatic sealer. Currently, optically clear heat seals are available. Chemically resistant foil seals will be added soon.
For plates that cannot be seal
ed a cover
instruction can be used in which a lid type is specified. This instruction is completed by the gantry system.
Updated over 3 years ago