Liquid Handling - Multi Channel (Stamping)
Multi-channel liquid handling at Strateos supports full 96-tip (8x12) as well as full rows (8xN) and full columns (Nx12) transfer in the SBS-compliant 96-tip format using the stamp
instruction.
Instruction and Parameters
The stamp
instruction has the field groups
, which is an array of transfer
objects
{
"op": "stamp",
"groups": [
{
"transfer": [
{
"from": origin,
"to": origin,
"volume": volume,
// same mix options as a 'transfer' group in the 'pipette' instruction
"mix_before": {...}, //optional
"mix_after": {...} //optional
},
{...} // additional transfers using same tips
],
"shape": { // default: {"rows": 8, "columns": 12}
"rows": integer,
"columns": integer
},
"tip_layout" : integer, // optional, default: 96
}
]
}
Each transfer
takes a volume
of liquid from
wells of a plate and transfers it to
another plate using whole rows or whole columns of tips dependent on the shape
.
The from
and to
wells correspond to the top-left wells of the source and destination respectively.
The shape
parameter describes a rectangle with values rows
and columns
that specify the number of rows
and columns
of tips to be transferred in accordance to the SBS-compatible tip_layout
. Strateos currently only supports tip_layout = 96
and either rows
must be 8 or columns
must be 12 (only full rows or full columns can be transferred).
Each transfer
must have the same shape
and tip_layout
Please be aware that tip_layout = 96
will address a 384 well plate by skipping every other row and column.
Every transfer
uses new tips. Instructions within a transfer
use the same tips.
Volume Range | %CV |
---|---|
<5μL | 2.1% |
5μL - 200μL | 1% |
200μL - 1000μL | 1% or less |
Tips
The tips used for stamp are automatically selected based on the total volume being moved in a single operation. Transfers within the same stamp
group must use the same tip size.
Tip size | Maximum volume | Minimum volume |
---|---|---|
Small | 31μL | 1μL |
Large | 110μL | 31μL |
Device
Multi-channel liquid handling operations are performed using an Agilent Bravo liquid handler.
Updated over 3 years ago