Pipetting Tools - Plate Stamp
Description
This protocol will transfer liquid from a full plate or individual plate quadrants to a new or existing container, enabling reformatting between 96-well and 384-well plates.
Sample requirements
| Sample | Explanation |
|---|---|
| Source container | A container of liquid sample that you wish to reformat into a new container. |
| Destination plate (from inventory) | A container that you wish to transfer sample into from your source container. |
Required parameters
Stamp uses
stampStamp uses a multichannel pipette to perform this liquid handling operation. To learn more about multichannel liquid handling and the
stampcommand please see here.
| Parameter | Explanation |
|---|---|
| Stamp Groups | A stamp group defines all parameters for a single stamp operation. Additional stamp operations may be specified by clicking the 'Add Stamp Groups' link at the bottom of the stamp group. |
| Source Quadrant | The portion of the source container to be reformatted (see below). |
| Transfer volume | The volume from the selected quadrant to be reformatted. |
| Destination | Select either a new container or a container from your inventory to contain the transfer volume from the source quadrant. New containers can be of type 96-pcr, 96-flat, 96-deep, 384-pcr, 384-flat, or 384-echo.From your inventory select a container. |
| New plate name (new container) | The name for the resulting new container |
| Container type (new container) | The container type for the new container. Select from 96-pcr, 96-flat, 96-deep, 384-pcr, 384-flat or 384-echo. |
| Storage condition | The storage temperature you require for the destination container: either '-80 C', '-20 C', '4 C' or 'Room Temperature'. |
| Destination Quadrant | The portion of the destination container into which the specified volume of the source quadrant should be stamped (see below). |
Source and Destination Quadrants
Choices for plate quadrants are Full plate, A1, A2, B1, and B2. A 96-well plate only contains one quadrant, so that Full plate and A1 for a 96-well plate are equivalent. In a 384-well plate, A1 refers to the upper left 8 row x 12 column quadrant. A2 is the quadrant to the right of this, and B1 and B2 specify the left and right quadrants on the bottom of the plate, respectively.
Protocol outputs
This protocol outputs a either a new container or an existing container with the specified volume of liquid stamped from the source quadrant to the destination quadrant. New containers will be stored in your Inventory at the specified storage condition. Existing containers will be returned to the storage condition from which they came.
No measurement data is returned by this protocol.
Recommended guidelines
Ensure you are aware of the dead volume in the source container. Dead volumes can be found in the containers section here. Failure to take into account dead volumes can result in insufficient volume being stamped.
Further reading
Check out the forum if you have more questions.
For further advice on your protocol, please ask questions to the community at https://forum.transcriptic.com
Updated almost 3 years ago