Pipetting Tools - Dilute

Description

This protocol takes a volume of an aliquot and dilutes it into a new container with a choice of diluent from the Strateos reagent library or from your own inventory.

Sample requirements

Sample

Explanation

Sample to dilute

An aliquot of a liquid sample that you wish to have diluted

Diluent (from inventory)

An aliquot of a liquid sample that you wish to use to dilute your other sample

Required parameters

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Dilute uses transfer

Dilute uses a single channel pipette to perform the liquid handling. To learn more about single channel liquid handling and the transfer command please see here

Parameter

Explanation

Sample (group)

A sample group defines all parameters for a single dilution. Additional dilutions may be specified by clicking the 'Add Sample' link at the bottom of the Sample group.

Container label

The name for the new resulting diluted aliquot. Aliquot names associated with this container will be derived from the source aliquot name.

Volume of sample

The volume of sample to be diluted.

Volume of diluent

The volume of diluent to be added to the volume of sample to be diluted.

Tube type for diluted aliquot

The new container for the newly diluted aliquot. Compatible containers: micro-1.5, micro-2.0, 96-pcr, 96-deep, 96-flat, 384-pcr, 384-flat, 384-echo.

Storage condition for diluted aliquot

The storage temperature you require for the diluted sample: either cold_20, cold_4 or ambient.

Diluent

Select either a diluent from the reagent library or from your inventory.

From the library both water and TE Buffer are available.

From your inventory select an aliquot.

Protocol outputs

This protocol outputs a new aliquot of the diluted sample which is stored in your inventory at your specified storage condition. Any aliquots used in the making of the dilutions will be returned to the storage condition from which they came.

No measurement data is returned by this protocol.

Recommended guidelines

Ensure you are aware of the dead volume in the containers you are using for both the sample to be diluted and the diluent (if you are using a diluent from your inventory). Dead volumes can be found in the containers section here. Failure to take into account dead volumes can result in insufficient volume being pipetted.